The Museo delle Mura ("museum of the walls") is an archaeological museum in Rome, Italy. It is housed in the first and second floors of the Porta San Sebastiano at the beginning of the Appian Way. It provides an exhibition on the walls of Rome and their building techniques, as well as the opportunity to walk along the inside of one of the best-preserved stretches of the Aurelian Wall.[2] The museum is free of charge.

The museum in its present form, was officially opened in 1990. Prior to 1939, the Porta San Sebastiano (also known as the Porta Appia) had been open to the public but it was then taken over by Ettore Muti, the Secretary of the Italian Fascist Party.[3] White-and-black mosaics in some rooms date back to that time. From 1970, there was a small museum connected to the internal parapet of the Aurelian Wall but this museum was only open to the public on Sundays, and, after a few years, was closed.[4]